Oxigraf Model O2Bio Oxygen and CO2 Gas Analyzers

Fast Gas Analyzer for Bio-Physical Applications and Bioreactors   

Based on the Oxigraf Model O2Cap Oxygen and CO2 analyzer, the O2Bio is specifically designed to provide gas monitoring of Bioreactor headspace and off-gas streams. The unit will measure continuously from an off-gas vent or periodically via a gas sample valve to take small 10-15ml samples from the bioreactor headspace via keypad or digital interface control.

The O2Bio integrates an Oxigraf tunable diode laser (TDL) oxygen sensor and a non-dispersive infrared (NDIR) CO2 sensor with a pump regulated gas sampling system. The analyzer features a bright vacuum fluorescent alphanumeric display (VFD), touch panel keypad, a rear 0-1VDC analog BNC output for O2 readings, rear terminal strip with limit detection relays and 4 – 20 mA analog outputs of O2 and CO2 reading, and a RS232 digital interface.

O2Bio – Bioreactor Off-Gas Monitoring of O2 and CO2

All inputs and outputs are CPC quick connect fittings with O-ring seals. Gas Inlet and Outlet with Swagelok or Luer fittings can be provided optionally.

The Oxigraf sensor uses laser diode absorption technology to measure oxygen concentration in the gas sample. A laser diode produces light in the visible spectrum at 760 nanometers. Light at this wavelength is absorbed by oxygen. To analyze oxygen the laser beam is focused through the sample gas onto a detector.  Oxygen concentration is inversely proportional to the amount of light reaching the detector. An analysis is made every 10 ms.

The analyzer automatically zeroes at each measurement interval by electronically tuning the laser to oxygen non-absorption wavelength. CO2 is measured using a NDIR based accessory sensor and integrated into our hardware.

Oxygen measurements are made independent of sample pressure, gas temperature, and (in XC mode) other gases including Ar, He, H₂, CO₂ and H₂O. Gases other than oxygen will not affect the measurement except for their dilution effect on the gas mixture.

APPLICATIONS:

• Fast Gas Analysis of: Oxygen and CO₂
• Overlay and Sparge Gas Controller for Bioreactors
• Microbial Systems & Biofuel Development
• Biomass Growth Rate and Substrate Consumption Monitoring
• Anaerobic Fermentation
• Stem Cell & Mammalian Cell Cultures
  

Gas Sampling and Valve Control:

The O2Bio/O2Bio-B Analyzers are specifically designed to provide gas monitoring of Bioreactor headspace and off-gas streams. The unit will measure continuously from an off-gas vent or periodically via a gas sample valve to take small 10-15ml samples from the bioreactor headspace via keypad or digital interface control.

This Oxigraf analyzer is equipped with a solenoid sampling valve with 1 ms response time enabling sampling of very small sample volumes.

PERFORMANCE SPECIFICATIONS:

• Range:                      0 to 10% CO2; 5 to 100% Oxygen
• Resolution:              0.1% in 0 to 100% O2 range; 0.01% in 0 to 10% CO2 range
• Sample Flow:          50 to 250 ml/min pump on; 50 to 500 ml/min pump off.
• Response Time:     Approximately 150 ms @ 250 ml/min flow (depending on electronic filter setting).
• Valve Response:    1 ms for Solenoid sampling valve
• Stability (4hrs):      ±0.2%.CO2 after 5 minute warm up; ±0.3%oxygen in XC mode, ±0.1% in oxygen LN mode
• Dimensions:            7.5 x 3.0 x 14.0 inches (190 x 76 x 356 mm) WxHxD
• Weight:                     7 pounds (3.2 kg)
• Warranty:                One year

Back Panel Interface:

Back Panel O2Bio with rear terminal strip with limit detection relays:

The power on/off switch (Press “I” for on, “O” for off), 12 VDC power input jack, service interface, terminal block interface, and a gas outlet port are located on the instrument back panel.  Power is supplied by an external 12 Volt, 2 Amp CSA Level 3 power supply. The RS – 232 port is provided to interface with a computer.

Calibration Kit:

Calibration Kit includes certified concentration gas cylinders, preset flow regulators, and tubing with connectors which mate with the analyzer and gas cylinder. The tubing assembly includes a fine flow needle valve. Instrumentation grade, certified calibration gas meets FDA standards for USP oxygen analyzers. Each cylinder provides approximately 3 months of daily calibrations. Calibration gas is shipped direct from the gas supplier.

Please download your Oxigraf Model O2Bio/O2Bio-B Oxygen and CO2 Gas Analyzers Brochure (pdf)